ADMINISTRATION AND DOSAGE
The vaccine can be administered via in-ovo or subcutaneous injection. In-ovo injection for embryonated broiler chicken eggs at 18 days of incubation: the dose of rehydrated vaccine is 0.05 ml (i.e. 2,000 doses reconstituted with 100 ml of diluent or 5,000 doses reconstituted with 250 ml of diluent). Subcutaneous injection for day-old chicks: the dose of rehydrated vaccine is 0.1 ml (i.e. 1,000 doses reconstituted with 100 ml of diluent or 2,500 doses reconstituted with 250 ml of diluent). Dissolve completely the freeze-dried contents of the vial in a volume of diluent that complies with the size of the packaging and the route of administration (in–ovo or s.c.).

In-ovo injection

The embryos should be injected at 18 days of age; a convenient time is when the egg is transferred from the setting to the hatching trays. Prior to injection, check that the position of the egg is correct (long axis vertical, with the air cell pointing up). Only use automatic injection devices that are designed specifically for in ovo injection. After injection, the eggs should be transferred to the hatching trays without delay so as to avoid harming the embryos due to chilling.
 
Subcutaneous injection
This injection should be performed appropriately under the skin of the neck of the bird using an automatic or manual injection device.